![]() ![]() UPMC Magee-Womens Imaging (West Mifflin) UPMC Outpatient Center 275 Clairton Bld. ![]() A referral from your provider is required. Hours: Monday – Friday 8:00am – 4:00pm (last patient appt at 4pm) We accept most insurances. Chemical Engineering, and Imaging offers four B.S. The practitioner's primary taxonomy code is 2085R0202X. The NPI number of this provider is 1528153665 and was assigned on October 2006. The membrane is immediately scanned using an imager following manufacturer’s instructions.Western imaging Western Imaging, Marina Del Rey is a provider established in Marina Del Rey, California operating as a Radiology with a focus in diagnostic radiology. ![]() The antibodies are visualized by mixing the chemiluminescence development substrates, Peroxide Buffer and Luminol/Enhancer Solution (Pierce), in a 1:1 ratio and incubating this mixture on the membrane for 5 minutes.For chemiluminescence detection, the secondary antibody, horseradish peroxidase-conjugated α-mouse IgG, is incubated with the membrane at a 1:10,000 dilution in TBST for 1 hour.The reactions are terminated by washing the membranes in water. The antibodies are visualized by adding the color development substrates, Nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolylphosphate (BCIP), in alkaline phosphatase buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris-HCl, pH 9.5). ![]()
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